integrin alpha9 / alpha5 EGFP-N3
(Plasmid #13603)

• Depositing Lab: Dean Sheppard
• Publication: Other integrin alpha9 plasmids (unpublished)

Backbone

• Vector backbone: EGFP-N3
• Backbone manufacturer: BD Biosciences
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: integrin alpha 9 / alpha 5
• Alt name: ITGA9
• Species: H. sapiens (human)
• Insert Size (bp): 3200
• Mutation: Integrin alpha 9 chimera containing the cytoplasmic domain of integrin alpha 5
• GenBank ID: NM_002207
• Entrez Gene: ITGA9 (a.k.a. ALPHA-RLC, ITGA4L, RLC)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CMV Forward primer
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see Young et al. (2001), MBC 12:3214 for more information regarding the construction of this chimera.