pcDNA3.1(+)_UG7
(Plasmid
#135990)
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PurposeExpresses UG7 in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 135990 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUtrCH
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Alt nameUtrophin
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SpeciesH. sapiens (human)
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Insert Size (bp)1464
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GenBank ID
- Promoter CMV promoter
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Tag
/ Fusion Protein
- helical linker-mEGFP
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV
- 3′ sequencing primer BGH (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+)_UG7 was a gift from Sumio Terada (Addgene plasmid # 135990 ; http://n2t.net/addgene:135990 ; RRID:Addgene_135990) -
For your References section:
Genetically encoded orientation probes for F-actin for fluorescence polarization microscopy. Nakai N, Sato K, Tani T, Saito K, Sato F, Terada S. Microscopy (Oxf). 2019 Jul 2. pii: 5525243. doi: 10.1093/jmicro/dfz022. 10.1093/jmicro/dfz022 PubMed 31264686
