epi-ABEmax-NG
(Plasmid
#135976)
-
Purpose(Empty Backbone) Expresses ABEmax(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 135976 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneepiCRISPR plasmid
-
Modifications to backboneContains D10A/R1335V/L1111R/D1135V/G1218R/E1219F/A1322R/T1337R mutations within ABEmax(with SpCas9-NG)
-
Vector typeMammalian Expression
-
Selectable markersBlasticidin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
epi-ABEmax-NG was a gift from Yongming Wang (Addgene plasmid # 135976 ; http://n2t.net/addgene:135976 ; RRID:Addgene_135976) -
For your References section:
Base Editing Mediated Generation of Point Mutations Into Human Pluripotent Stem Cells for Modeling Disease. Qi T, Wu F, Xie Y, Gao S, Li M, Pu J, Li D, Lan F, Wang Y. Front Cell Dev Biol. 2020 Sep 25;8:590581. doi: 10.3389/fcell.2020.590581. eCollection 2020. 10.3389/fcell.2020.590581 PubMed 33102492