KO plasmid
(Plasmid
#135970)
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Purpose(Empty Backbone) Expresses SpCas9, and cloning backbone for sgRNA
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 135970 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneepiCRISPR plasmid
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
KO plasmid was a gift from Yongming Wang (Addgene plasmid # 135970 ; http://n2t.net/addgene:135970 ; RRID:Addgene_135970) -
For your References section:
krCRISPR: an easy and efficient strategy for generating conditional knockout of essential genes in cells. Wang B, Wang Z, Wang D, Zhang B, Ong SG, Li M, Yu W, Wang Y. J Biol Eng. 2019 Apr 24;13:35. doi: 10.1186/s13036-019-0150-y. eCollection 2019. 10.1186/s13036-019-0150-y PubMed 31049076
