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Addgene

gRNAU6
(Plasmid #135963)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135963 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    gRNA_Cloning Vector
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    gRNAU6 was a gift from Yongming Wang (Addgene plasmid # 135963 ; http://n2t.net/addgene:135963 ; RRID:Addgene_135963)

  • For your References section:

    An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells. Xie Y, Wang D, Lan F, Wei G, Ni T, Chai R, Liu D, Hu S, Li M, Li D, Wang H, Wang Y. Sci Rep. 2017 May 24;7(1):2320. doi: 10.1038/s41598-017-02456-y. 10.1038/s41598-017-02456-y PubMed 28539611
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