epiCRISPRe
(Plasmid
#135962)
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Purpose(Empty Backbone) Expresses SpCas9, and cloning backbone for sgRNA
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 135962 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneepiCRISPR plasmid
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
K848A/K1003A/R1060A
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
epiCRISPRe was a gift from Yongming Wang (Addgene plasmid # 135962 ; http://n2t.net/addgene:135962 ; RRID:Addgene_135962) -
For your References section:
An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells. Xie Y, Wang D, Lan F, Wei G, Ni T, Chai R, Liu D, Hu S, Li M, Li D, Wang H, Wang Y. Sci Rep. 2017 May 24;7(1):2320. doi: 10.1038/s41598-017-02456-y. 10.1038/s41598-017-02456-y PubMed 28539611