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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13596 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBabe puro
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Backbone manufacturerAvailable at Addgene (plasmid #1764)
- Backbone size w/o insert (bp) 5169
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameintegrin beta 6
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Alt nameITGB6
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SpeciesH. sapiens (human)
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Insert Size (bp)2400
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GenBank IDNM_000888
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Entrez GeneITGB6 (a.k.a. AI1H)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI/SnaBI (destroyed during cloning)
- 3′ cloning site XbaI/SnaBI (destroyed during cloning)
- 5′ sequencing primer pBabe 5' (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Although the SnaBI cloning site was destroyed, there is a BamHI site 5' of the insert and EcoRI and SalI sites 3' of the insert.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
integrin beta6 pBabe puro was a gift from Dean Sheppard (Addgene plasmid # 13596 ; http://n2t.net/addgene:13596 ; RRID:Addgene_13596)