pCS2+8 Flag2AUF1(p42)
(Plasmid #135946)

• Purpose: Expresses AUF1p42 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.
• Depositing Lab: Yimon Aye
• Publication: Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 10.1096/fj.201901930R.

Backbone

• Vector backbone: pCS2+8
• Backbone size w/o insert (bp): 4134
• Total vector size (bp): 5207
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: heterogeneous nuclear ribonucleoprotein D
• Alt name: AUF1 p42
• Species: H. sapiens (human)
• Insert Size (bp): 1073
• GenBank ID: NM_031369.2
• Entrez Gene: HNRNPD (a.k.a. AUF1, AUF1A, HNRPD, P37, hnRNPD0)
• Promoter: CMV and SP6
• Tag / Fusion Protein:
  • Flag2 (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV forward or SP6 forward
• 3′ sequencing primer: SV40 reverse

Resource Information

• Supplemental Documents:
  • 2plus8p42.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry