pET28a His6HuR
(Plasmid #135943)

• Purpose: Expresses HuR in E. coli
• Depositing Lab: Yimon Aye
• Publication: Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 10.1096/fj.201901930R.

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 5234
• Total vector size (bp): 6242
• Modifications to backbone: Thrombin cleavage site (between His tag and HuR) has been replaced with TEV cleavage site
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ELAV like RNA binding protein 1
• Alt name: HuR, ELAVL1
• Species: H. sapiens (human)
• Insert Size (bp): 1050
• GenBank ID: NM_001419.3
• Entrez Gene: ELAVL1 (a.k.a. ELAV1, HUR, Hua, MelG)
• Promoter: T7
• Tag / Fusion Protein:
  • His6 (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: T7 reverse

Resource Information

• Supplemental Documents:
  • pET28a HuR.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry