pET28a His6Flag2AUF1(p37)
(Plasmid
#135939)
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PurposeExpresses AUF1p37 in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 135939 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
- Backbone size w/o insert (bp) 5255
- Total vector size (bp) 6176
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameheterogeneous nuclear ribonucleoprotein D
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Alt nameAUF1, HNRNPD p37 isoform
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SpeciesH. sapiens (human)
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Insert Size (bp)926
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GenBank IDheterogeneous nuclear ribonucleoprotein D
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Entrez GeneHNRNPD (a.k.a. AUF1, AUF1A, HNRPD, P37, hnRNPD0)
- Promoter T7
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Tags
/ Fusion Proteins
- His6 (N terminal on insert)
- Flag2 (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 forward
- 3′ sequencing primer T7 reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a His6Flag2AUF1(p37) was a gift from Yimon Aye (Addgene plasmid # 135939 ; http://n2t.net/addgene:135939 ; RRID:Addgene_135939) -
For your References section:
Post-transcriptional regulation of Nrf2-mRNA by the mRNA-binding proteins HuR and AUF1. Poganik JR, Long MJC, Disare MT, Liu X, Chang SH, Hla T, Aye Y. FASEB J. 2019 Nov 1:fj201901930R. doi: 10.1096/fj.201901930R. 10.1096/fj.201901930R PubMed 31665914