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pSPL3-ABCC8-2
(Plasmid #135916)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135916 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSPL3
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 6031
  • Total vector size (bp) 5740
  • Modifications to backbone
    We cut 953 bp and inserted 668 bp encoding the exon 2 of ABCC8
  • Vector type
    Mammalian Expression ; Exon trapping

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATP-binding Cassette subfamily C member 8
  • Alt name
    ABCC8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1027
  • GenBank ID
    NM_001351296.2
  • Entrez Gene
    ABCC8 (a.k.a. ABC36, HHF1, HI, HRINS, MRP8, PHHI, PNDM3, SUR, SUR1, SUR1delta2, TNDM2)
  • Promoter SV40

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer CTCGAGggggaggtaccagcatagga
  • 3′ sequencing primer GCTAGCaaggtaactccggcaggttg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSPL3-ABCC8-2 was a gift from Diana Valverde Pérez (Addgene plasmid # 135916 ; http://n2t.net/addgene:135916 ; RRID:Addgene_135916)

  • For your References section:

    Characterization of rare ABCC8 variants identified in Spanish pulmonary arterial hypertension patients. Lago-Docampo M, Tenorio J, Hernandez-Gonzalez I, Perez-Olivares C, Escribano-Subias P, Pousada G, Baloira A, Arenas M, Lapunzina P, Valverde D. Sci Rep. 2020 Sep 15;10(1):15135. doi: 10.1038/s41598-020-72089-1. 10.1038/s41598-020-72089-1 PubMed 32934261
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