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integrin alpha9 / alpha4 pcDNA1 neo
(Plasmid #13587)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13587 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNAI neo
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Growth instructions
    Plasmid contains SupF. Grow in TOP10/P3 cells.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    integrin alpha 9 / alpha 4
  • Alt name
    ITGA9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3200
  • Mutation
    Integrin alpha 9 chimera containing the cytoplasmic domain of integrin alpha 4
  • GenBank ID
    NM_002207
  • Entrez Gene
    ITGA9 (a.k.a. ALPHA-RLC, ITGA4L, RLC)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See article for cloning details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    integrin alpha9 / alpha4 pcDNA1 neo was a gift from Dean Sheppard (Addgene plasmid # 13587 ; http://n2t.net/addgene:13587 ; RRID:Addgene_13587)

  • For your References section:

    The cytoplasmic domain of the integrin alpha9 subunit requires the adaptor protein paxillin to inhibit cell spreading but promotes cell migration in a paxillin-independent manner. Young BA, Taooka Y, Liu S, Askins KJ, Yokosaki Y, Thomas SM, Sheppard D. Mol Biol Cell. 2001 Oct . 12(10):3214-25. 10.1091/mbc.12.10.3214 PubMed 11598204
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