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Addgene

integrin alpha9 pcDNA1 neo
(Plasmid #13585)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13585 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNAI neo
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Growth instructions
    Plasmid contains SupF. Grow in TOP10/P3 cells.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    integrin alpha 9
  • Alt name
    ITGA9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3200
  • GenBank ID
    NM_002207
  • Entrez Gene
    ITGA9 (a.k.a. ALPHA-RLC, ITGA4L, RLC)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the first 41 amino acids of the integrin alpha insert show greater identity to integrin alpha-5 rather than integrin alpha-9

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    integrin alpha9 pcDNA1 neo was a gift from Dean Sheppard (Addgene plasmid # 13585 ; http://n2t.net/addgene:13585 ; RRID:Addgene_13585)

  • For your References section:

    The integrin alpha 9 beta 1 mediates cell attachment to a non-RGD site in the third fibronectin type III repeat of tenascin. Yokosaki Y, Palmer EL, Prieto AL, Crossin KL, Bourdon MA, Pytela R, Sheppard D. J Biol Chem. 1994 Oct 28. 269(43):26691-6. PubMed 7523411
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