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Addgene

integrin beta6 777T pcDNA1 neo
(Plasmid #13582)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13582 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNAI neo
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Growth instructions
    Plasmid contains SupF. Grow in TOP10/P3 cells.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    integrin beta 6 777T
  • Alt name
    ITGB6
  • Alt name
    integrin beta 6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2400
  • Mutation
    777T. Lacks last 11 amino acids of cytoplasmic domain.
  • GenBank ID
    NM_000888
  • Entrez Gene
    ITGB6 (a.k.a. AI1H)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    integrin beta6 777T pcDNA1 neo was a gift from Dean Sheppard (Addgene plasmid # 13582 ; http://n2t.net/addgene:13582 ; RRID:Addgene_13582)

  • For your References section:

    Loss of integrin alpha(v)beta6-mediated TGF-beta activation causes Mmp12-dependent emphysema. Morris DG, Huang X, Kaminski N, Wang Y, Shapiro SD, Dolganov G, Glick A, Sheppard D. Nature. 2003 Mar 13. 422(6928):169-73. 10.1038/nature01413 PubMed 12634787
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