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Addgene

pTACR TH1-p2aGFP
(Plasmid #135815)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135815 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    px330
  • Backbone size w/o insert (bp) 8484
  • Total vector size (bp) 9405
  • Modifications to backbone
    added p2a MYR eGFP cloned TH spacer into BBSI site
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TH 1 guide
  • Tag / Fusion Protein
    • p2a Myr-eGFP

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTACR TH1-p2aGFP was a gift from Tim Ahfeldt & Lee Rubin (Addgene plasmid # 135815 ; http://n2t.net/addgene:135815 ; RRID:Addgene_135815)

  • For your References section:

    Pathogenic Pathways in Early-Onset Autosomal Recessive Parkinson's Disease Discovered Using Isogenic Human Dopaminergic Neurons. Ahfeldt T, Ordureau A, Bell C, Sarrafha L, Sun C, Piccinotti S, Grass T, Parfitt GM, Paulo JA, Yanagawa F, Uozumi T, Kiyota Y, Harper JW, Rubin LL. Stem Cell Reports. 2019 Dec 20. pii: S2213-6711(19)30444-8. doi: 10.1016/j.stemcr.2019.12.005. 10.1016/j.stemcr.2019.12.005 PubMed 31902706
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