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Addgene

Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
(Plasmid #135812)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135812 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDestTol2pA2AC
  • Vector type
    zebrafish

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TagRFP
  • Alt name
    TagRFP
  • Species
    Synthetic
  • Insert Size (bp)
    735
  • GenBank ID
    ZDB-EFG-110425-1
  • Promoter UAS

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP was a gift from Nadia Mercader Huber (Addgene plasmid # 135812 ; http://n2t.net/addgene:135812 ; RRID:Addgene_135812)
  • For your References section:

    Wilms Tumor 1b Expression Defines a Pro-regenerative Macrophage Subtype and Is Required for Organ Regeneration in the Zebrafish. Sanz-Morejon A, Garcia-Redondo AB, Reuter H, Marques IJ, Bates T, Galardi-Castilla M, Grosse A, Manig S, Langa X, Ernst A, Piragyte I, Botos MA, Gonzalez-Rosa JM, Ruiz-Ortega M, Briones AM, Salaices M, Englert C, Mercader N. Cell Rep. 2019 Jul 30;28(5):1296-1306.e6. doi: 10.1016/j.celrep.2019.06.091. 10.1016/j.celrep.2019.06.091 PubMed 31365871