pPS833
(Plasmid
#1358)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1358 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPS293
- Backbone size w/o insert (bp) 5200
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGSP1
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Alt nameYepGAL GSP1 G21V
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SpeciesS. cerevisiae (budding yeast)
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MutationG21V, stabilizes the GTP-bound form
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Entrez GeneGSP1 (a.k.a. YLR293C, CNR1, CST17)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pPS293 has the GAL1 promoter (EcoRI/XbaI fragment) in YEp352 (2 micron ori).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPS833 was a gift from Pamela Silver (Addgene plasmid # 1358 ; http://n2t.net/addgene:1358 ; RRID:Addgene_1358) -
For your References section:
The GTP-bound form of the yeast Ran/TC4 homologue blocks nuclear protein import and appearance of poly(A)+ RNA in the cytoplasm. Schlenstedt G, Saavedra C, Loeb JD, Cole CN, Silver PA. Proc Natl Acad Sci U S A 1995 Jan 3;92(1):225-9. 10.1073/pnas.92.1.225 PubMed 7816822