-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13577 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepTrc99
-
Backbone manufacturerPharmacia
- Backbone size w/o insert (bp) 4000
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameRat liver fatty acid binding protein
-
Alt nameFABP1
-
SpeciesR. norvegicus (rat)
-
Insert Size (bp)564
-
GenBank IDBC086947
-
Entrez GeneFabp1 (a.k.a. Fabplg, L-FABP, SCP, SCP., p14)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTrc99A-Rat FABP1 was a gift from Martin Scanlon (Addgene plasmid # 13577 ; http://n2t.net/addgene:13577 ; RRID:Addgene_13577) -
For your References section:
An improved method for the purification of rat liver-type fatty acid binding protein from Escherichia coli. Velkov T, Chuang S, Prankerd R, Sakellaris H, Porter CJ, Scanlon MJ. Protein Expr Purif. 2005 Nov . 44(1):23-31. 10.1016/j.pep.2005.04.006 PubMed 15914028