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Addgene

XLone-ETV2
(Plasmid #135763)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135763 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    XLone
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ETV2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1029
  • Mutation
    none
  • GenBank ID
    NM_014209
  • Entrez Gene
    ETV2 (a.k.a. ER71, ETSRP71)
  • Promoter TRE3G

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer M13 FWD
  • 3′ sequencing primer M13 REV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XLone-ETV2 was a gift from Xiaojun Lian (Addgene plasmid # 135763 ; http://n2t.net/addgene:135763 ; RRID:Addgene_135763)

  • For your References section:

    Direct programming of human pluripotent stem cells into endothelial progenitors with SOX17 and FGF2. Ream MW, Randolph LN, Jiang Y, Chang Y, Bao X, Lian XL. Stem Cell Reports. 2024 Mar 5:S2213-6711(24)00044-4. doi: 10.1016/j.stemcr.2024.02.006. 10.1016/j.stemcr.2024.02.006 PubMed 38518781
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