pFETCh_ZNF264_iso1
(Plasmid
#135731)
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PurposeDonor vector for 3' FLAG tag of human ZNF264_iso1
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 135731 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFETCh_Donor
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZNF264_iso1 Homology arms
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SpeciesH. sapiens (human)
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Entrez GeneZNF264
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Tag
/ Fusion Protein
- 3XFLAG-P2A-NeoR (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFETCh_ZNF264_iso1 was a gift from Richard M. Myers (Addgene plasmid # 135731 ; http://n2t.net/addgene:135731 ; RRID:Addgene_135731) -
For your References section:
CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts BS, Mackiewicz M, Mendenhall EM, Myers RM. Genome Res. 2015 Sep 9. 10.1101/gr.193540.115 PubMed 26355004