pLI50
(Plasmid #13573)

• Purpose: (Empty Backbone)
• Depositing Lab: Chia Lee
• Publication: Lee et al Gene. 1991 Jul 15. 103(1):101-5.

Backbone

• Vector backbone: N/A
• Backbone size (bp): 5505
• Vector type: S. aureus-E. coli shuttle vector
• Selectable markers: Chloramphenicol in S. aureus at 37C

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: N/A

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that a 81bp deletion was detected by Addgene upstream of the CmR gene compared to the depositor's reference sequence. The depositor confirmed that this was present in their original stock and does not impact CmR expression or the overall function of the plasmid.