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pRSET FLIPglu-600uDelta11 Ares-Aphrodite
(Plasmid #13571)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13571 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSET-B
  • Backbone size w/o insert (bp) 2897
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLIPglu F16A Delta11 Aphrodite
  • Alt name
    Ares-mglB-Aphrodite
  • Alt name
    Periplasmic glucose binding
  • Species
    E. coli
  • Insert Size (bp)
    2400
  • Mutation
    Fluorophore-derived linker shortened. F16A mutation in mglB
  • GenBank ID
    DQ525024 DQ525025
  • Tags / Fusion Proteins
    • Ares (N terminal on insert)
    • Aphrodite (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html

EYFP was exchanged with Aphrodite (accession number DQ525025), which is an codon-diversified version of Venus/EYFP. Ares (accession number DQ525024) was exchanged for ECFP, which is an codon-diversified version of ECFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET FLIPglu-600uDelta11 Ares-Aphrodite was a gift from Wolf Frommer (Addgene plasmid # 13571 ; http://n2t.net/addgene:13571 ; RRID:Addgene_13571)

  • For your References section:

    Rapid Metabolism of Glucose Detected with FRET Glucose Nanosensors in Epidermal Cells and Intact Roots of Arabidopsis RNA-Silencing Mutants. Deuschle K, Chaudhuri B, Okumoto S, Lager I, Lalonde S, Frommer WB. Plant Cell. 2006 Sep . 18(9):2314-25. 10.1105/tpc.106.044073 PubMed 16935985
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