pRSET FLIPglu-600uDelta13 Aphrodite
(Plasmid #13570)

• Depositing Lab: Wolf Frommer
• Publication: Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Backbone

• Vector backbone: pRSET-B
• Backbone size w/o insert (bp): 2897
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FLIPglu F16A Delta13 Aphrodite
• Alt name: ECFP-mglB-Aphrodite
• Alt name: Periplasmic glucose binding
• Species: E. coli
• Insert Size (bp): 2400
• Mutation: Fluorophore-derived linker shortened. F16A mutation in mglB
• GenBank ID: DQ525025
• Tags / Fusion Proteins:
  • ECFP (N terminal on insert)
  • Aphrodite (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html

EYFP was exchanged with Aphrodite (accession number DQ525025), which is an codon-diversified version of Venus/EYFP.