pMB22.SV40.Zeo
(Plasmid
#135692)
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PurposeLentivirus delivery for stable expression of AsCas12a-VPR, Zeocin selection
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 135692 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLL3.3
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Backbone manufacturerD. Tschaharganeh
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMB22.SV40.Zeo was a gift from Florian Karreth (Addgene plasmid # 135692 ; http://n2t.net/addgene:135692 ; RRID:Addgene_135692)