cTRE-GFP-miR30_shPten.1523
(Plasmid
#135667)
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PurposeIntroduce Dox-inducible (TRE promoter) GFP cDNA plus miR30-embedded shPten into (ES) cells by recombination-mediated cassette exchange
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 135667 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepgkATGfrt
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Backbone manufacturerRudolf Jaenisch (Addgene plasmid # 20734)
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Vector typeMouse Targeting
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameshPten
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gRNA/shRNA sequenceunknown
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GFP; shRNA embedded in miR30
Please note there are 2 mutations-V163A, F165L in GFP. Depositor confirms this does not affect functionality of GFP.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
cTRE-GFP-miR30_shPten.1523 was a gift from Florian Karreth (Addgene plasmid # 135667 ; http://n2t.net/addgene:135667 ; RRID:Addgene_135667)
