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Addgene

cTRE-GFP-miR30_shRen
(Plasmid #135666)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135666 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pgkATGfrt
  • Backbone manufacturer
    Rudolf Jaenisch (Addgene plasmid # 20734)
  • Vector type
    Mouse Targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    shRenilla
  • gRNA/shRNA sequence
    unknown
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GFP; shRNA embedded in miR30

Please note there are 2 mutations-V163A, F165L in GFP. Depositor confirms this does not affect functionality of GFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cTRE-GFP-miR30_shRen was a gift from Florian Karreth (Addgene plasmid # 135666 ; http://n2t.net/addgene:135666 ; RRID:Addgene_135666)
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