pBS L30 Flag-Apex-Rab7A
(Plasmid #135652)

• Purpose: Rab7A fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.
• Depositing Lab: Raphael Gaudin
• Publication: Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7803. doi: 10.1126/sciadv.aba7803. Print 2021 Jan.

Backbone

• Vector backbone: pBS
• Modifications to backbone: Modified for mammalian expression using the promoter of the mouse ribosomal protein L30 gene ("weak promoter") and a HSV TK polyA tail
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Rab7A
• Alt name: Rab7
• Alt name: ras-related protein Rab-7a
• Species: H. sapiens (human)
• Insert Size (bp): 624
• GenBank ID: NP_004628.4 7879
• Entrez Gene: RAB7A (a.k.a. CMT2B, PRO2706, RAB7)
• Promoter: L30
• Tags / Fusion Proteins:
  • FLag (N terminal on insert)
  • Apex2 (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13pUC-fwd
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry