pRSET FLII12Pglu-600u
(Plasmid #13563)

• Depositing Lab: Wolf Frommer
• Publication: Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Backbone

• Vector backbone: pRSET-B
• Backbone size w/o insert (bp): 2897
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FLIPglu F16A Y12S-ECFP-D13R
• Alt name: mglB with ECFP inserted
• Alt name: Periplasmic glucose binding
• Species: E. coli
• Insert Size (bp): 2400
• Mutation: F16A ECFP insertion in binding protein
• Tag / Fusion Protein:
  • Citrine (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Citrine is from Roger Tsien, UCSD.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html

Citrine is modified version of EYFP.