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Addgene

pET51b-NAD-Luc
(Plasmid #135627)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135627 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET51-b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5218
  • Total vector size (bp) 7159
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NAD-Luc sensor
  • Insert Size (bp)
    2097
  • Promoter T7
  • Tags / Fusion Proteins
    • Strep (N terminal on insert)
    • Hisx10 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET51b-NAD-Luc was a gift from Kai Johnsson (Addgene plasmid # 135627 ; http://n2t.net/addgene:135627 ; RRID:Addgene_135627)

  • For your References section:

    A biosensor for measuring NAD(+) levels at the point of care. Yu Q, Pourmandi N, Xue L, Gondrand C, Fabritz S, Bardy D, Patiny L, Katsyuba E, Auwerx J, Johnsson K. Nat Metab. 2019 Dec;1(12):1219-1225. doi: 10.1038/s42255-019-0151-7. Epub 2019 Dec 13. 10.1038/s42255-019-0151-7 PubMed 32694678
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