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pFastBac SpyTag-β2AR-SpyTag
(Plasmid #135620)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135620 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac
  • Backbone size w/o insert (bp) 5693
  • Total vector size (bp) 6794
  • Modifications to backbone
    Flag tag on N terminus, eGFP-10xHis-R1D4 tag on C terminus have been added.
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Beta 2 adrenergic receptor
  • Alt name
    B2AR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1101
  • Mutation
    Deleted 1-24 aa, 366-413 aa
  • Entrez Gene
    ADRB2 (a.k.a. ADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR)
  • Tags / Fusion Proteins
    • Spytag (N terminal on insert)
    • Spytag (C terminal on insert)
    • eGFP-10xHis-R1D4 tag (C terminal on backbone)
    • HA-FLAG tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Cla1 (not destroyed)
  • 3′ cloning site Nhe1 (not destroyed)
  • 5′ sequencing primer GGA TTA TTC ATA CCG TCC CA
  • 3′ sequencing primer CGT CGC CGT CCA GCT CGA CCA G
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac SpyTag-β2AR-SpyTag was a gift from Hee-Jung Choi (Addgene plasmid # 135620 ; http://n2t.net/addgene:135620 ; RRID:Addgene_135620)

  • For your References section:

    Watching helical membrane proteins fold reveals a common N-to-C-terminal folding pathway. Choi HK, Min D, Kang H, Shon MJ, Rah SH, Kim HC, Jeong H, Choi HJ, Bowie JU, Yoon TY. Science. 2019 Nov 29;366(6469):1150-1156. doi: 10.1126/science.aaw8208. 10.1126/science.aaw8208 PubMed 31780561
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