pscAAV-Tyr
(Plasmid #135616)

• Purpose: Donor vector for genomic targeting of an EcoRI restriction site to the mouse Tyrosinase locus
• Depositing Lab: Lin He
• Publication: Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e4. doi: 10.1016/j.celrep.2019.05.103.

Backbone

• Vector backbone: pscAAV
• Backbone size w/o insert (bp): 4836
• Total vector size (bp): 5794
• Modifications to backbone: Contains 1 kb stuffer sequence 5' of the insert
• Vector type: Mouse Targeting, AAV, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: EcoRI restriction site flanked by Tyr homology arms
• Alt name: Tyrosinase
• Species: M. musculus (mouse)
• Insert Size (bp): 958
• GenBank ID: 22173
• Entrez Gene: Tyr (a.k.a. Oca1, albino, c, skc35)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pscAAV_Tyr.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry