pSP64-H2B-GFP
(Plasmid
#135605)
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PurposeExpression of H2B-GFP mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 135605 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
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Vector typeIn vitro transcription (mRNA synthesis)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH2B-GFP
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SpeciesH. sapiens (human), Synthetic
- Promoter Unknown
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSP64-H2B-GFP was a gift from Mamiko Yajima (Addgene plasmid # 135605 ; http://n2t.net/addgene:135605 ; RRID:Addgene_135605) -
For your References section:
An optogenetic approach to control protein localization during embryogenesis of the sea urchin. Uchida A, Yajima M. Dev Biol. 2018 Sep 1;441(1):19-30. doi: 10.1016/j.ydbio.2018.06.015. Epub 2018 Jun 26. 10.1016/j.ydbio.2018.06.015 PubMed 29958898