pSP64-Lifeact-GFP-ePDZ
(Plasmid
#135602)
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PurposeExpression of Lifeact-GFP-ePDZ mRNA for microinjection to be used for optogenetics in S. purpuratus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 135602 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSP64
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Vector typeIn vitro transcription (mRNA synthesis)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLifeact-GFP-ePDZ
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SpeciesSynthetic; S. purpuratus
- Promoter Unknown
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Tags
/ Fusion Proteins
- EGFP (C terminal on insert)
- ePDZ (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSP64-Lifeact-GFP-ePDZ was a gift from Mamiko Yajima (Addgene plasmid # 135602 ; http://n2t.net/addgene:135602 ; RRID:Addgene_135602) -
For your References section:
An optogenetic approach to control protein localization during embryogenesis of the sea urchin. Uchida A, Yajima M. Dev Biol. 2018 Sep 1;441(1):19-30. doi: 10.1016/j.ydbio.2018.06.015. Epub 2018 Jun 26. 10.1016/j.ydbio.2018.06.015 PubMed 29958898