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Addgene

pSP64-Lifeact-GFP
(Plasmid #135601)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135601 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP64
  • Vector type
    In vitro transcription (mRNA synthesis)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lifeact-GFP
  • Species
    Synthetic; S. purpuratus
  • Promoter Unknown
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSP64-Lifeact-GFP was a gift from Mamiko Yajima (Addgene plasmid # 135601 ; http://n2t.net/addgene:135601 ; RRID:Addgene_135601)

  • For your References section:

    An optogenetic approach to control protein localization during embryogenesis of the sea urchin. Uchida A, Yajima M. Dev Biol. 2018 Sep 1;441(1):19-30. doi: 10.1016/j.ydbio.2018.06.015. Epub 2018 Jun 26. 10.1016/j.ydbio.2018.06.015 PubMed 29958898
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