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Addgene

pSP64-Vasa-GFP
(Plasmid #135595)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135595 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP64
  • Vector type
    In vitro transcription (mRNA synthesis)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vasa-GFP
  • Species
    S. purpuratus
  • Promoter SP6
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSP64-Vasa-GFP was a gift from Mamiko Yajima (Addgene plasmid # 135595 ; http://n2t.net/addgene:135595 ; RRID:Addgene_135595)

  • For your References section:

    Essential elements for translation: the germline factor Vasa functions broadly in somatic cells. Yajima M, Wessel GM. Development. 2015 Jun 1;142(11):1960-70. doi: 10.1242/dev.118448. Epub 2015 May 14. 10.1242/dev.118448 PubMed 25977366
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