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Addgene

pG.Lantern-INS-SM50-GFP
(Plasmid #135594)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135594 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGreenLantern2
  • Vector type
    In vitro transcription (mRNA synthesis)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    INS-SM50 promoter
  • Alt name
    SM50
  • Entrez Gene
    SM50
  • Promoter CMV
  • Tag / Fusion Protein
    • Green Lantern GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pG.Lantern-INS-SM50-GFP was a gift from Koji Akasaka & Mamiko Yajima (Addgene plasmid # 135594 ; http://n2t.net/addgene:135594 ; RRID:Addgene_135594)

  • For your References section:

    Ars insulator protects transgenes from long-term silencing in sea urchin larva. Yajima M, Kiyomoto M, Akasaka K. Dev Genes Evol. 2007 Apr;217(4):331-6. doi: 10.1007/s00427-007-0140-9. Epub 2007 Mar 20. 10.1007/s00427-007-0140-9 PubMed 17372754
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