pG.Lantern-SM50-GFP
(Plasmid
#135593)
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PurposeExpression of SM50 Promoter-GFP mRNA for microinjection to visualize SM50 promoter activity in S. purpuratus
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 135593 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGreen Lantern 2
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Vector typeIn vitro transcription (mRNA synthesis)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSM50 promoter
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SpeciesS. purpuratus
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Entrez GeneSM50
- Promoter CMV
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Tag
/ Fusion Protein
- Green Lantern GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pG.Lantern-SM50-GFP was a gift from Koji Akasaka & Mamiko Yajima (Addgene plasmid # 135593 ; http://n2t.net/addgene:135593 ; RRID:Addgene_135593) -
For your References section:
Ars insulator protects transgenes from long-term silencing in sea urchin larva. Yajima M, Kiyomoto M, Akasaka K. Dev Genes Evol. 2007 Apr;217(4):331-6. doi: 10.1007/s00427-007-0140-9. Epub 2007 Mar 20. 10.1007/s00427-007-0140-9 PubMed 17372754