pGL3-Basic-hCYP26A1P-E4-luciferase
(Plasmid #135592)

• Purpose: Short form DNA fragment of human CYP26A1gene promoter (E4) in pGL3-Basic-luc vector
• Depositing Lab: Catharine Ross
• Publication: Zhang et al Gene. 2010 Sep 15;464(1-2):32-43. doi: 10.1016/j.gene.2010.05.004. Epub 2010 Jun 8.

Backbone

• Vector backbone: pGL3-Basic-luciferase
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4818
• Total vector size (bp): 5130
• Selectable markers: None

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Follow the protocol from Promega
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Short form promoter of human CYP26A1 gene (E4)
• Alt name: hCYP26A1P (E4)
• Species: H. sapiens (human)
• Insert Size (bp): 312
• Promoter: Human CYP26A1 promoter (Short Form, E4)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: AGCAAGCTTGTACAGATAGATTAAAACGT
• 3′ sequencing primer: AATAAGCTTCACGAAGGTGCAGAGCGC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry