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Addgene

pJPS2953
(Plasmid #135587)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135587 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSE358
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    snR6
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    U6Δ5 has AUUUC at position 103–107 deleted
  • Entrez Gene
    snR6 (a.k.a. snR6)
  • Promoter Native

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid is based on pSX6 that is deposited in Addgene. pSX6 was used as the parental plasmid for site directed mutagenesis to introduce internal deletions with U6

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJPS2953 was a gift from Jonathan Staley (Addgene plasmid # 135587 ; http://n2t.net/addgene:135587 ; RRID:Addgene_135587)

  • For your References section:

    Termination of pre-mRNA splicing requires that the ATPase and RNA unwindase Prp43p acts on the catalytic snRNA U6. Toroney R, Nielsen KH, Staley JP. Genes Dev. 2019 Sep 26. pii: gad.328294.119. doi: 10.1101/gad.328294.119. 10.1101/gad.328294.119 PubMed 31558568
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