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pFastBac-Dual-M2
(Plasmid #135584)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135584 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac-Dual-M2
  • Backbone manufacturer
    pFastBac-dual
  • Backbone size (bp) 5201
  • Modifications to backbone
    An 80 bp cis-element was inserted upstream of AcMNPV polyhedrin promoter for higher protein expression.
  • Vector type
    Insect Expression ; transfer vector
  • Promoter polyhedrin of AcMNPV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac-Dual-M2 was a gift from Xiao-Wen Cheng (Addgene plasmid # 135584 ; http://n2t.net/addgene:135584 ; RRID:Addgene_135584)

  • For your References section:

    Improved pFastBac donor plasmid vectors for higher protein production using the Bac-to-Bac(R) baculovirus expression vector system. Shang H, Garretson TA, Kumar CMS, Dieter RF, Cheng XW. J Biotechnol. 2017 Aug 10;255:37-46. doi: 10.1016/j.jbiotec.2017.06.397. Epub 2017 Jun 20. 10.1016/j.jbiotec.2017.06.397 PubMed 28645582
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