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pOTTC1535 pscAAV mU6 shRNA(scram) CMV-IE Nuc-EYFP
(Plasmid #135563)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135563 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pscAAV CMV-IE Nuc-EYFP
  • Backbone manufacturer
    NIDA OTTC
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Mammalian Expression, AAV, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    shRNA (scrambled)
  • Alt name
    scrambled
  • Species
    M. musculus (mouse), R. norvegicus (rat)
  • Insert Size (bp)
    -1
  • Promoter mU6

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Nuc-EYFP
  • Alt name
    Yellow Fluorescent protein
  • Insert Size (bp)
    800
  • Promoter CMV-IE
  • Tag / Fusion Protein
    • 3xNLS (C terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOTTC1535 pscAAV mU6 shRNA(scram) CMV-IE Nuc-EYFP was a gift from Christopher Richie (Addgene plasmid # 135563 ; http://n2t.net/addgene:135563 ; RRID:Addgene_135563)

  • For your References section:

    Abstinence-dependent dissociable central amygdala microcircuits control drug craving. Venniro M, Russell TI, Ramsey LA, Richie CT, Lesscher HMB, Giovanetti SM, Messing RO, Shaham Y. Proc Natl Acad Sci U S A. 2020 Apr 7;117(14):8126-8134. doi: 10.1073/pnas.2001615117. Epub 2020 Mar 23. 10.1073/pnas.2001615117 PubMed 32205443
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