Skip to main content
Addgene

pFastBac HT JS-Munc18b
(Plasmid #135554)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135554 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pFastBac
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Munc18b
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1782
  • Entrez Gene
    Stxbp2 (a.k.a. Munc18b)
  • Promoter polyhedrin promoter
  • Tag / Fusion Protein
    • 6xHis tag and TEV site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CAGTTTTGTAATAAAAAAACCTATAAAT
  • 3′ sequencing primer TAAGCTGCAATAAACAAGTT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac HT JS-Munc18b was a gift from Jingshi Shen (Addgene plasmid # 135554 ; http://n2t.net/addgene:135554 ; RRID:Addgene_135554)

  • For your References section:

    Functional Reconstitution of Intracellular Vesicle Fusion Using Purified SNAREs and Sec1/Munc18 (SM) Proteins. Yu H, Crisman L, Stowell MHB, Shen J. Methods Mol Biol. 2019;1860:237-249. doi: 10.1007/978-1-4939-8760-3_15. 10.1007/978-1-4939-8760-3_15 PubMed 30317509
Related items:
Commonly requested with: