pET-28a-His6-SUMO-VAMP8
(Plasmid
#135553)
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PurposeExpress mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 135553 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVAMP8
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SpeciesM. musculus (mouse)
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Insert Size (bp)303
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MutationNo ATG start codon
- Promoter T7 promoter
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Tag
/ Fusion Protein
- 6xHis tag, thrombin recognition and cleavage site, then ubiquitin-like protein tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-28a-His6-SUMO-VAMP8 was a gift from Jingshi Shen (Addgene plasmid # 135553 ; http://n2t.net/addgene:135553 ; RRID:Addgene_135553) -
For your References section:
Functional Reconstitution of Intracellular Vesicle Fusion Using Purified SNAREs and Sec1/Munc18 (SM) Proteins. Yu H, Crisman L, Stowell MHB, Shen J. Methods Mol Biol. 2019;1860:237-249. doi: 10.1007/978-1-4939-8760-3_15. 10.1007/978-1-4939-8760-3_15 PubMed 30317509
Map uploaded by the depositor.
