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pET-28a-His6-SUMO-VAMP2
(Plasmid #135551)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135551 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VAMP2
  • Insert Size (bp)
    354
  • Mutation
    No ATG start codon
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • 6xHis tag, thrombin recognition and cleavage site, then ubiquitin-like protein tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-28a-His6-SUMO-VAMP2 was a gift from Jingshi Shen (Addgene plasmid # 135551 ; http://n2t.net/addgene:135551 ; RRID:Addgene_135551)

  • For your References section:

    Functional Reconstitution of Intracellular Vesicle Fusion Using Purified SNAREs and Sec1/Munc18 (SM) Proteins. Yu H, Crisman L, Stowell MHB, Shen J. Methods Mol Biol. 2019;1860:237-249. doi: 10.1007/978-1-4939-8760-3_15. 10.1007/978-1-4939-8760-3_15 PubMed 30317509
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