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Addgene

pRSET FLIPPi-4u
(Plasmid #13553)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13553 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSET-B
  • Backbone size w/o insert (bp) 2897
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLIPPi T163A
  • Alt name
    ECFP-PiBP-EYFP
  • Alt name
    Phosphate binding protein
  • Species
    Synechococcus
  • Insert Size (bp)
    2400
  • Mutation
    T163A in binding protein
  • Tags / Fusion Proteins
    • ECFP (N terminal on insert)
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET FLIPPi-4u was a gift from Wolf Frommer (Addgene plasmid # 13553 ; http://n2t.net/addgene:13553 ; RRID:Addgene_13553)

  • For your References section:

    A novel analytical method for in vivo phosphate tracking. Gu H, Lalonde S, Okumoto S, Looger LL, Scharff-Poulsen AM, Grossman AR, Kossmann J, Jakobsen I, Frommer WB. FEBS Lett. 2006 Oct 30. 580(25):5885-93. 10.1016/j.febslet.2006.09.048 PubMed 17034793
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