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pCEP4-myc-H2-Dd-VN (Y84C/C121S/A139C)
(Plasmid #135501)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135501 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10381
  • Total vector size (bp) 11989
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2-Dd
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1608
  • Mutation
    Y84C/C121S/A139C
  • GenBank ID
    U47326.1
  • Entrez Gene
    H2-D1 (a.k.a. H-2D, H2-D, H2-K1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Signal peptide from hemagglutinin (N terminal on insert)
    • Extracellular c-myc epitope tag (N terminal on insert)
    • VN: Venus (I152L) N-terminal residues 1-154 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV_fwd
  • 3′ sequencing primer EBV_rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4-myc-H2-Dd-VN (Y84C/C121S/A139C) was a gift from Erik Procko (Addgene plasmid # 135501 ; http://n2t.net/addgene:135501 ; RRID:Addgene_135501)

  • For your References section:

    Molecular determinants of chaperone interactions on MHC-I for folding and antigen repertoire selection. McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG. Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 10.1073/pnas.1915562116. Epub 2019 Dec 3. 10.1073/pnas.1915562116 PubMed 31796585
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