pPS1070
(Plasmid #1355)

• Depositing Lab: Pamela Silver
• Publication: Seedorf et al Proc Natl Acad Sci U S A 1997 Aug 5;94(16):8590-5.

Backbone

• Vector backbone: pPS934
• Backbone size w/o insert (bp): 7300
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: KAP123
• Alt name: pPS934-KAP123-GFP
• Alt name: KAP123
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: KAP123 (a.k.a. YER110C, YRB4)
• Tag / Fusion Protein:
  • sGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: M13R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

KAP123 replaces NUF2 of pPS934, CEN plasmid.