pGI3EM09
(Plasmid #135482)

• Purpose: Agrobacterium T-DNA with SpunHsp70pr driving hph-GFP
• Depositing Lab: Nicolas Buchler
• Publication: Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 10.7554/eLife.52741.

Backbone

• Vector backbone: pGI3
• Backbone size w/o insert (bp): 9369
• Total vector size (bp): 12417
• Modifications to backbone: HindII/EcoRI digestion for cloning insert
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Used in Agrobacterium EHA105 for transformation
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SpunHsp70pr hph-GFP ADH1t
• Species: S. cerevisiae (budding yeast); Spizellomyces punctatus
• Promoter: SpunHsp70pr
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: gtaaaacgacggccagt
• 3′ sequencing primer: caggaaacagctatgac

Resource Information

• Supplemental Documents:
  • pGI3EM09.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The pGI3 vector was a gift from Giuseppe Ianiri