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p2,0_3M5M_eGFP
(Plasmid #135474)

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Item Catalog # Description Quantity Price (USD)
Plasmid 135474 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p2,0
  • Backbone manufacturer
    Andrew Ball, University of Alabama at Birmingham
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Marburg virus minigenome, eGFP reporter
  • Species
    Marburg marburgvirus, Musoke variant
  • Mutation
    None
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2,0_3M5M_eGFP was a gift from Elke Mühlberger (Addgene plasmid # 135474 ; http://n2t.net/addgene:135474 ; RRID:Addgene_135474)

  • For your References section:

    A Chimeric Lloviu Virus Minigenome System Reveals that the Bat-Derived Filovirus Replicates More Similarly to Ebolaviruses than Marburgviruses. Manhart WA, Pacheco JR, Hume AJ, Cressey TN, Deflube LR, Muhlberger E. Cell Rep. 2018 Sep 4;24(10):2573-2580.e4. doi: 10.1016/j.celrep.2018.08.008. 10.1016/j.celrep.2018.08.008 PubMed 30184492
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