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Addgene

pCAGGS_VP30_MARV
(Plasmid #135472)

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Item Catalog # Description Quantity Price (USD)
Plasmid 135472 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VP30
  • Species
    Marburg marburgvirus, Musoke variant
  • Mutation
    None
  • Promoter AG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI/StuI (destroyed during cloning)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

VP30 gene flanked by BamHI sites

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS_VP30_MARV was a gift from Elke Mühlberger (Addgene plasmid # 135472 ; http://n2t.net/addgene:135472 ; RRID:Addgene_135472)

  • For your References section:

    A Chimeric Lloviu Virus Minigenome System Reveals that the Bat-Derived Filovirus Replicates More Similarly to Ebolaviruses than Marburgviruses. Manhart WA, Pacheco JR, Hume AJ, Cressey TN, Deflube LR, Muhlberger E. Cell Rep. 2018 Sep 4;24(10):2573-2580.e4. doi: 10.1016/j.celrep.2018.08.008. 10.1016/j.celrep.2018.08.008 PubMed 30184492
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